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Microbiology



Location -

  • OPD 15, 1st floor, B wing
  • Practical class and departmental office: Old casualty building, ground floor

Email ID -

Contact Us -

  • Extension of Bacteriology and Serology lab- 206
  • Extension of Office – 341

Teaching Faculty

Non Teaching Staff

Activities of the Department

UG
(To be filled by the administrative office)
  1. Intake capacity of UG with status of recognition
  2. List of admitted students, merit and category wise for 2019-20; 2020-21
PG
  1. Intake capacity of PG with status of recognition: Nil
  2. List of admitted students, merit and category wise for 2019-20; 2020-21: Nil
Research publications through the department in 2019-20; 2020-21 (mandatory) and since inception (desirable) in Vancouver style of referencing.
  • Samal B, Bisure K, Shastri J. Trichosporon beigelli infection a child with congenital extrophy bladder. Indian Pract [Internet]. 2015;68(12):43–4. Available from: https://medtechasia.in/articles/index.php/tip/article/view/519
  • Mehta RS, Koticha AH, Kuyare SS, Mehta PR. Are We Neglecting Blastocystis Hominis in Patients Having Irritable Bowel Syndrome. J Evol Med Dent Sci. 2015;4(64):11164–71.
  • Gupta R, Mehta P, Koticha A, Kuyare S, Ingole N, Hase N. Intestinal parasitic infections in renal allograft recipients – necessity of periodic screening and prudent choice of diagnostic techniques – a prospective study. Int J Curr Microbiol Appl Sci [Internet]. 2015;4(10):757–63. Available from: http://www.ijcmas.com/vol-4-10/Rajarshi Gupta, et al.pdf
  • Shinde S, Koticha A, Mehta P, Gatne M. Subconjunctival dirofilariasis. J Case Reports. 2015;5(2):340–2.
  • Meshram R, Gedam D. Prevalence of Hepatitis B Virus Infection in General Population of Nagpur Region. J Contemp Med Dent. 2015;3(2):48–50.
  • Phulsunge S, Koticha A, Shinde S, Mehta P. Intestinal Parasites in Patients Having Haematological Malignancies. J Evol Med Dent Sci. 2016;5(31):1613–7.
  • Kuyare SS, Koticha AH, Mehta PR. Strongyloides Stercoralis and Its Risk Factors: an Experience At a Tertiary Care Hospital. J Evol Med Dent Sci. 2016;5(50):3199–202.
  • Paranjpe S, Koticha A, Mehta PR. Chronic giardiasis in a case of common variable immunodeficiency (CVID): A case report. J Clin Diagnostic Res. 2016;10(7):DD03–4.
  • Malpekar K, Vivek K, Shastri J. Clinical Correlation and Laboratory Diagnosis of Bacterial Vaginosis. J Evol Med Dent Sci. 2016;5(60):4148–53.
  • Mukhopadhyay S, Malpekar K, Shastri J. Intestinal Parasitic and Bacterial Infection Among Food Handlers in a Metropolitan Tertiary Care Hospital. J Evol Med Dent Sci. 2016;5(62):4327–31.
  • Thorat S S, Kulkarni B R. Occurrence and detection of AmpC beta-lactamases among clinical isolates of enterobacteriaceae in a tertiary care hospital, Bagalkot; using three phenotypic methods. J Evol Med Dent Sci. 2016;5(94):6940–8.
  • Chavan SP, Meshram RB, Raut SS, Vedi JN. A Rare Case of Retropharyngeal Abscess by Salmonella Typhimurium in Central India. Ann Int Med Dent Res. 2016;2(6):1–3.
  • Sawant S, Sonawane A, Shastri J. Prevalence of hepatitis A & E among sporadic cases of acute hepatitis from a tertiary care hospital of Western India. Int J Curr Res. 2016;8(03):28673–6.
  • Suryawanshi RS, Wanjare SW, Koticha AH, Mehta PR. Onychomycosis: dermatophytes to yeasts: an experience in and around Mumbai, Maharashtra, India. Int J Res Med Sci. 2017;5(5):1959.
  • Verma S, Thorat S S, Bisure K. Prevalence of carbapenem resistant Enterobacteriaceae from a tertiary care hospital in Mumbai, India. J Microbiol Infect Dis. 2013;03(04):207–10.
  • Paranjpe SM, Koticha AH, Mehta PR. Swelling on the Arm: An Unusual Presentation of Microfilaria. J Case Reports. 2018;8(4):271–4.
  • Akale PS, Paranjpe SM, Koticha AH, Mehta PR. Prevalence of Intestinal Parasites in Alcoholics. J Evol Med Dent Sci. 2019;8(33):2607–11.
  • Meshram R, Gajbhiye S, Raut S. Study of organisms causing osteomyelitis in atertiary care hospital. J Evolution Med Dent Sci. 2020
  • Meshram R, Gajbhiye S, Raut S. Biofilm production by the organisms causing osteomyelitis in a tertiary care hospital. Annala of International Medical nad Dental Research, 2019
Additional courses with list of students:

Nil

Other Academic activities (including timetables)
  • Lecture timing: Monday and Wednesday – 9-10 am Saturday – 8-9 am
  • Practical timing: Tuesday and Thursday- 1-4pm
  • No. of MBBS students per batch- 150
  • Theory lectures- 111
  • Out of these 111 lectures, 68 lectures were taken online.
  • Practicals and demonstrations- 32 (cards for practical demonstration have been made)
  • Tutorials- 12
  • Museum exhibits
    • Models- 12
    • Charts- approx 50
    • Others- approx 90
  • Library – 143 books
  • Teaching of nursing students and training of DMLT students are also done.
  • Biomedical Waste Management trainings are being carried out by the Department.
  • Faculty is a part of the Hospital Infection Control Committee and Biomedical Waste Management Committee.
Research activities
  • Knowledge and practices of hand hygiene among health care workers in our hospital
  • Utility of clot culture in diagnosis of enteric fever
  • Assessment of antiHBS titers in subjects after full course vaccination- under which the first and second year MBBS students have been vaccinated against hepatitis B
  • Study of hospital microbiota and the effect of cleaning
  • Study of microbiota of accessory used by health care worker including stethoscope and mobile phone.
Awards and Achievements received by students and faculty since inception:

Nil

Results for examination for the year

Degree/Diploma Year No of Students Appeared No of Students Passed Passing percentage
UG MBBS Winter 2019 153 148 96.7
PG PG
PG
2019-2020
2020-2021
NIL
NIL
—–
—–
—–
—–
Other information
Sections/Units of the department
  • Bacteriology
  • Serology
  • Immunology
  • Mycology
  • Parasitology
  • Mycobacteriology, RNTCP
  • ICTC
  • Hospital infection control committee
  • Molecular biology
  • BMW (advisory committee)
  • COVID-19 lab
Time and days:

Days Time
Routine lab Monday to Saturday 8:30 am to 3:30 pm
Emergency lab Monday to Saturday 3:30 pm to 8: 30 am
Emergency lab Sunday and OPD holiday 24 hours
COVID-19 related activities
  • HOD Microbiology has been appointed as the Dean of Dahisar Kandarpada jumbo COVID facility.
  • Training of postgraduate residents, interns MBBS and nursing students were taken regarding infection prevention, biomedical waste management and specimen collection
  • SOP for rational use of PPE were made and a video tutorial showing right way of donning and doffing of PPE was also made by department for training of hospital staff.
  • Training imparted to other technicians from HBTH Jogeshwari and Kandar pada Jumbo COVID facility for specimen transport and rapid antigen testing.
  • Design for isolation ward was made for setting up a COVID isolation ward.
  • In this ongoing COVID-19 pandemic, the department is accepting swabs for covid testing throughout the day from 23/03/2020.
  • Though, all the swabs were sent to outside laboratory for PCR testing initially, 6 July 2020 onwards rapid antigen testing for SARS CoV-2 was also started. This testing is also done in emergency laboratory. The results are available within half an hour.
  • SARS CoV 2 Antigen testing
  • On 22nd July 2020, CBNAAT testing for SARS CoV-2 was also started. This testing is done for hospitalized patients, suspected of COVID-19 or having other comorbidities.
  • SARS Cov-2 CBNAAT testing
  • Till date more than 10000 samples have been sent to outside lab for PCR. More than 4000 rapid antigen tests and more than 1000 CBNAAT tests have been performed till date.
  • In the coming days, the department is going to start COVID antibody ELISA testing for hospital staff. This will be for surveillance purpose.

CME

Details of following activities since inception (with photos wherever possible)
CME, workshops, training:
  • Along with the Department of Medicine, a CME on needle stick injuries was conducted in March 2017. A lecture on Universal safety precautions and Biomedical Waste management was conducted.
  • BMW Trainings for MBBS students, Nursing staff, Laboratory staff and Teaching faculty was conducted by the Department of Microbiology. Lecture on update of new rules was taken in December 2017.
  • Recent trends in diagnosis of Drug resistant Tuberculosis talk was delivered by faculty in RNTCP(PMDT) training held in March 2018.
  • The department conducted its first CME on “Surveillance of HAI and the concept of bundle care.”

Vision of Department

  • Department aims at delivering quality reports for which all teaching and non-teaching staff are working together
  • Faculty is getting trained for Infection Control Surveillance so as to impart quality service for HICC.
  • Automated systems (ID/AST) are also being procured especially for patients where timely and early reports are necessary.
  • Academic section aims at imparting quality knowledge through small group teachings, student seminars, role plays.
Services provided (new and old)

Sr. no. Section Services offered
1 Bacteriology
  • • Microscopy, culture and antimicrobial susceptibility testing of aerobic bacteria
  • • Hanging drop of stool for V. cholera
  • • Albert’s stain of throat swab for C. diphtheriae
  • • Microscopy and culture of anaerobic bacteria
2 Serology
  • RA, ASO
  • CRP
  • Widal
  • Rapid Dengue NS1, Dengue IgM/ IgG, Leptospirosis IgM
  • Rapid HBsAg, HCV antibody
  • RPR, TPHA
3 Immunology
  • • ELISA for HBsAg, HCV antibody, HAV antibody, HEV antibody, Dengue NS1 antigen and Dengue IgM antibody, Leptospirosis IgM antibody.
4 Mycology
  • Microscopy and culture of fungi
  • Slide culture for identification and speciation of fungi
  • India ink preparation of CSF for Cryptococcus
5 Parasitology
  • Routine and microscopic examination of stool sample, pus from liver abscess and blood for parasites
  • Special staining of stool samples for opportunistic parasites like Cryptosporidium, Cyclospora, Isospora and microsporidium
  • RDT for malarial antigen
6 Mycobacteriology, RNTCP
    Microscopy for Mycobacterium tuberculosis (both pulmonary and extrapulmonary samples) Rapid detection of Mycobacteria and Rifampicin resistance using CBNAAT /GenXpert system in pulmonary and extrapulmonary samples Microscopy for Mycobacterium leprae
7 ICTC
  • Integrated Counselling and Testing Services for HIV/AIDS
  • Prevention of Parent To Child Transmission services
8 H.I.C.C.
  • • Surveillance of operation theatres in Dr. RNCH and affiliated peripheral MCGM hospitals
  • • Surveillance of personnel for MRSA
  • • Sterility checking of blood bags from blood bank
  • • Outbreak investigations
9 Molecular Biology
  • H1N1 swab PCR
    Samples are accepted
  • PCR of Dengue
    Samples are accepted
  • PCR of Leptospirosis
    Samples are accepted
10 BMW (advisory committee)
  • Training activities
11 COVID-19 Lab
  • Covid swabs of indoor patients are tested in the department by CBNAAT.
  • Rapid covid antigen testing.
  • Covid swabs are sent to Kasturba for PCR.
  • Molecular Laboratory will be setup soon, the procurement of equipments is already in process.
Emergency lab

Sr. no. Section Tests done
1 Bacteriology
  • Stool for hanging drop in a suspected case of cholera
  • Pus/ muscle tissue for gas gangrene (Gram’s staining)
  • Throat swab in a suspected case of diphtheria (Albert’s stain)
  • Blood cultures (only received)
2 Mycology
  • India Ink of CSF for Cryptococcus
3 Serology
  • Rapid Dengue NS1
  • Rapid Dengue IgM
  • Rapid Leptospirosis IgM
  • RDT for malarial antigen
  • CRP
4 Molecular Biology
  • H1N1 swab for PCR (only received)
5 COVID-19 Lab
  • Rapid Covid antigen testing
  • Covid swabs for PCR (only received)
Patient related/ Non-academic Activities
  • Average number of tests done in a month in routine lab.
  • Average number of tests done in a month emergency lab.
  • Weekly IDSP reporting is done.
  • ICTC outdoor camps are conducted regularly.
  • Weekly reporting of operation theatre swabs of RNCH and peripheral MCGM hospitals in western suburbs.
  • Faculty is a part of the HICC Committee and will be formulating the protocols for audit in HICC.
  • HOD Microbiology is a part of the advisory committee of the newly formed BMW management body.
  • Along with Department of Community Medicine, vaccination against Hepatitis-B is initiated for all heath care workers of the hospital.
  • Biomedical Waste Management poster for the Hospital was designed by the Dept. of Microbiology.
  • Standard Operating Procedures for Operation Theatres made by KEM Hospital, were modified for local implementation by the Department.
  • SOP for disinfection in hospital settings have been made by the department.
  • Department caters to all peripheral hospitals in the western suburbs for Operation theatre sterility testing (swabs), specimens for culture and antimicrobial susceptibility testing.
  • HBsAg vaccination is solely done by Microbiology department. Till date 300 MBBS students, 55 residents, 15 technicians and 51 nurses have been vaccinated under this programme.

Gallery